Figure 4From: Regulation of P450 oxidoreductase by gonadotropins in rat ovary and its effect on estrogen productionProduction of estrone by co-transfection of wild-type or mutant POR and aromatase expression vectors in COS-7 cells. A and B: Amounts of estrone in the culture medium were detected by EIA at the indicated times and for the indicated contents of POR expression vector, with or without aromatase expression vector. The protein expression of POR, aromatase, and GAPDH after transfection with these amounts of vectors was detected by western blot analysis. C and D: COS-7 cells were transfected with wild-type POR or R457H or V492E mutant expression vectors (20 ng/well) with aromatase expression vector, at the indicated times. Data are means and SEM values (B) or SD values (D) of at least three assays, respectively.Back to article page