Figure 3From: Regulation of P450 oxidoreductase by gonadotropins in rat ovary and its effect on estrogen productionRegulation of POR and aromatase mRNA and protein levels by FSH. Granulosa cells isolated from immature DES-primed rats were cultured and treated with FSH (30 ng/ml) for the indicated times, and the total RNA or protein was extracted. A: RT-PCR was performed with the specific primer sets for POR, aromatase, and GAPDH. B: Results of real-time PCR are also shown. Data are the mean ± SEM values of at least three independent experiments. *, P < 0.01. C: Western blot analysis was performed with antibodies to POR, aromatase, and GAPDH, using the same lysates.Back to article page