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Figure 3 | Reproductive Biology and Endocrinology

Figure 3

From: Reduced developmental competence of immature, in-vitro matured and postovulatory aged mouse oocytes following IVF and ICSI

Figure 3

Blocked embryos derived from 24 hrs in-vitro aged oocytes after ICSI. Scattered DNA is observed in all embryos. Embryos 3a, 3c, and 3e are not associated with positive TUNEL assay (3b, 3d, 3f). Most embryos contain cell fragments, empty of chromatin. Embryo 3 g has some cells with clear apoptotic (Ap) events identified by the TUNEL assay (3h). Left panel: bright field. Right field: fluorescence images for DAPI and FITC to detect DNA and positive TUNEL.

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