Figure 2From: Reduced developmental competence of immature, in-vitro matured and postovulatory aged mouse oocytes following IVF and ICSIPronuclei formation at 4–5 and 8–9 hrs after ICSI of IVM, freshly ovulated and 13 hrs aged oocytes injected at different maturation stages. Oocytes were injected with a single sperm head. Oocytes were fixed and stained with DAPI at different times after injection. SC = sperm chromatin; EC = egg chromatin; PN = pronuclei; PB = polar body.Back to article page