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Table 1 Genebank accession numbers, product sizes and nucleotide sequence details of primers for RT-PCR analysis of mRNA expression of bovine TLR 1 to 10.

From: Toll-like receptor and antimicrobial peptide expression in the bovine endometrium

Gene Primer Sequence 5'>3' Product Size (bp) Accession No.
TLR1 Sense ACT TGG AAT TCC TTC TTC ACG A 176 NM_001046504
  Anti-sense GGA AGA CTG AAC ACA TCA TGG A   
TLR2 Sense GGT TTT AAG GCA GAA TCG TTT G 190 NM_174197
  Anti-sense AAG GCA CTG GGT TAA ACT GTG T   
TLR3 Sense GAT GTA TCA CCC TGC AAA GAC A 195 NM_001008664
  Anti-sense TGC ATA TTC AAA CTG CTC TGC T   
TLR4 Sense CTT GCG TAC AGG TTG TTC CTA A 153 NM_174198
  Anti-sense CTG GGA AGC TGG AGA AGT TAT G   
TLR5 Sense CCT CCT GCT CAG CTT CAA CTA T 172 AY634631
  Anti-sense TAT CTG ACT TCC ACC CAG GTC T   
TLR6 Sense CCT TGT TTT TCA CCC AAA TAG C 154 NM_001001159
  Anti-sense TAA GGT TGG TCC TCC AGT GAG T   
TLR7 Sense TCT TGA GGA AAG GGA CTG GTT A 205 DQ333225
  Anti-sense AAG GGG CTT CTC AAG GAA TAT C   
TLR8 Sense TAA CCT TCG GAA TGT CTC CAG T 232 NM_001033937
  Anti-sense GTG GGA AAT TCT GTT TCG ACT C   
TLR9 Sense CTG ACA CCT TCA GTC ACC TGA G 156 NM_183081
  Anti-sense TGG TGG TCT TGG TGA TGT AGT C   
TLR10 Sense ATG GTG CCA TTA TGA ACC CTA C 248 NM_001076918
  Anti-sense CAC ATG TCC CTC TGG TGT CTA A