Figure 2

Flow cytometric determination of apoptotic germ cells. Representative dot blot histograms of Gc-5spg cells cultured in control medium (left panel) and in medium ontaining 200 μM CAP during 48 h (right panel). On the x-axis the FL1 fluorescence (autofluorescence) and on the y-axis the FL3 fluorescence (for PI) are indicated. The population of apoptotic cells (with hypodiploid DNA, arrow) becomes clear after incubation with CAP.