Figure 6

StAR mRNA analysis in vivo and in vitro. StAR mRNA expression measured by kinetic RT-PCR on Day 16 and 22 of pregnancy and Day 3 post-partum. mRNA levels were normalized against L19 and are shown as the mean ± SEM for all groups (n = 3). There was a significant difference among groups (P < 0.05; one-way ANOVA). Values without shared notations differ at P < 0.05 (LSD test). (b) mRNA expression measured by kinetic RT-PCR on Day 16 of pregnancy following incubation without trophic support for 8 h or following treatment with recombinant rat TNFα (37.5 ng/ml). mRNA levels were normalized against L19 and are shown as mean ± SEM for all groups (n = 3). Asterisk denotes significant difference between control and treatment groups (P < 0.05; t-test).