TNFα mRNA expression. TNFα mRNA expression was assessed through kinetic RT-PCR on Day 16 and 22 of pregnancy and Day 3 post-partum. mRNA levels were normalized against L19 and are shown as mean ± SEM for all groups (n = 3). There was a significant difference between pregnancy and post-partum time-points (P < 0.05; one-way ANOVA). Values without shared notations differ at P < 0.001 (LSD test).