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Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: The expression of tumor necrosis factor-alpha, its receptors and steroidogenic acute regulatory protein during corpus luteum regression

Figure 1

TNF receptor 1 & 2 immunohistochemistry. Rat CL sections incubated with polyclonal goat anti-rat TNFR1 or TNFR2 antibody stained with DAB and counterstained with haematoxylin. TNFR1 protein expression was assessed on Days 16 (a) and 22 (b) of pregnancy and Day 3 (c) post-partum. Oocytes at all stages of follicular development were immunoreactive (d) The staining pattern and intensity were consistent between immunohistochemical runs (n = 4) and staining intensity was greatest in Day 3 post-partum sections (c). TNFR2 protein was also assessed on Days 16 (e) and 22 (f) of pregnancy and Day 3 post-partum (g). Immunoreactive-TNFR was observed across all time-points within the CL compartment and specifically luteal cells (lc). Oocytes at all stages of follicular development were immunoreactive (h). Sections treated in the absence of the primary antibody showed no non-specific immunostaining for TNFR1 (inset d) or TNFR2 (inset h). [Magnification 400×]

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