Figure 4
From: Prostasome-like vesicles stimulate acrosome reaction of pig spermatozoa
Fluorescence pattern of pig spermatozoa stained with FITC-PNA + PI for the assessment of acrosome status and sperm viability. Dead cells showing nuclear red PI fluorescence: C-D. Live cells without PI staining: A acrosome-reacted cells with uniform green FITC-PNA fluorescence of acrosome cap; B: acrosome-unreacted cells with no staining of acrosomal cap (original magnification ×1000).