MMP and TIMP location. (A): gelatin zymography (10% gel) and (B): reverse zymography (13% gel) on the same two CMP suspensions; lanes 1 and 3: CMP fluid phases; lanes 2 and 4: CMP cell phases. Both enzymes and inhibitors are almost exclusively present in the fluid phases. (C): gelatin zymography performed on the two buffy coats; lanes 1 and 4: fluid phase, lanes 2–3 and 5–6: duplex of cellular phase. (D): Reverse zymography. Lane 1: TIMP-1 standard (4 ng, 28.5 kDa), lane 2: TIMP-2 standard (4 ng, 21 kDa), lanes 3 and 5: fluid phase. Lanes 4 and 6: cellular phase. For the buffy coats, TIMPs and MMPs are present in the cellular phase.