Figure 4

Arachidonic acid release by BEND cells. ³H-arachidonic acid (0.25 μCi/ml) was applied to cells for 24 h, cells were washed and treated for one h with vehicle (C, control), Phorbol 12,13 dibutyrate (PD), Bromoenol lactone (B), Pyrroline-1 (P), PD + B or PD + P. Medium and lysed cells were harvested separately and % of total radioactivity released into the medium determined by scintillation counting. Treatments were performed in triplicate and repeated (n = 6). Data are expressed as means +/- SEM. Columns with different superscripts are significantly different (p < 0,05).