Figure 4

Double stain immunofluorescence for unphosphorylated connexin 43 and c-kit in myometrium during term labour. Immunofluorescence using (a) FITC labelled unphosphorylated connexin 43 (Green), (b) TRITc labelled KIT antibodies (Red) and (c) overlap of the images to reveal a complete match indicating that the unphosphorylated connexin 43 expressing cells also express KIT (Bars = 150 micrometres). This finding indicates that these cells are MICs. The distribution of KIT is even throughout the entire cell membrane.