Skip to main content

Table 1 Primer sequences for PCR cloning.

From: Cloning of somatolactin alpha, beta forms and the somatolactin receptor in Atlantic salmon: Seasonal expression profile in pituitary and ovary of maturing female broodstock

Primers for sequencing Atlantic salmon slr:
slrF1: 5'-CGTGCCAGAGATTCCCAATAAAGAGTCAAC-3'
slrR1: 5'-GCTTGTCTGTTCTCCTCTCCTCCTC-3'
slrF3: 5'-GACTTCTATGCACAGGTCAGCGATGTGACGC-3'
slrR2: 5'-GAAGATGATGAGCATGAGGAGAATGGCCACGC-3'
slrF7: 5'-CACAACAACCACCACACAAACCATGG-3'
slrR9: 5'-CAAGTAGAACAAGGCTGTCTGTGATG-3'
slr5RACErev: 5'-TCAGAGTGGAGCCGCACACAGTATTTGATCC-3'
slr3RACEfor: 5'-TACCTGTTCCCCACAACCCCCCTCCAAG-3'
Primers for sequencing of Atlantic salmon Slα:
SlαF1: 5'-CACCATGAACATGATGCAAGTCATGCAG-3'
SlαR1: 5'-GGGTGGGYATAGTTGTTCTCTAATGAAG-3'
Slα5RACErev: 5'-CACAAAGTATGATGTTGCCCTGCTCGTCCTTAC-3'
Slα3RACEfor: 5'-TGGACGATGATATGCTGACCACCTCCTACTAC-3'
Primers for sequencing of Atlantic salmon Slβ:
SlβF1: 5'-CACCATGGAGTGTCAGGATCC-3'
SlβR1: 5'-ACTGGAGGGACTCTGCTATAAAAG-3'
Slβ5RACErev: 5'-GTGCACGCGTTCCCTGTTTGAGTCAACG-3'
Slβ3RACEfor: 5'-GAGCCTGGCAGACTACCCTGTGCATTTTG-3'