Table 1 Primers used in quantitative real-time RT-PCR
Gene Name | Upstream Primer (5'-3') | Downstream Primer (5'-3') | PCR product size (bp) |
|---|---|---|---|
18S | TCTTAGCTGAGTGTCCCGCG | ATCATGGCCTCAGTTCCG A | 150 |
AKR7A2 | AACTGGACACGGCCTTCATG | CCTTGGTGGCAATTTTCACTCTG | 109 |
ALDH1A1 | ACCCCAGGAGTCACTCAAGG | ACTGTGGGCTGGACAAAGTAG | 149 |
BCAR3 | CCTGGAAATGCCACAGATCAC | CTTCATGCAGGAGTTTGCTGAA | 124 |
C1orf186 | TAGCTTGGATAGCTCCTGCAGTTC | CATTTTTTAGTTCTCCAGGGTCAGA | 101 |
CLIC4 | TCACCAAAACACCCAGAATCAA | ACCCCTCTCCAGTGCTTCATTA | 108 |
COL4A1 | CACGGGTACTCTTTGCTCTACGT | AAGGGCATTGTGCTGAACTTG | 101 |
COL4A2 | CATGCCCTTCCTGTACTGCAA | GATGTACTTGATCTCGTCCT | 133 |
CXCR4 | CATCAGTCTGGACCGCTACC | GCAAAGATGAAGTCGGGAATAGTC | 138 |
ER-α | CGGCATTCTACAGGCCAAATT | AGCGAGTCTCCTTGGCAGATT | |
FZD5 | GCTACCAGCCGTCCTTCAGT | GAAGCGTTCCATGTCGATGAG | 128 |
KLF4 | CTGCGGCAAAACCTACACAAA | GAATTTCCATCCACAGCCGT | 106 |
KLF9 | TGGCTGTGGGAAAGTCTATGG | CTCGTCTGAGCGGGAGAACT | |
LAMC2 | GATGGCATTCACTGCGAGAAG | TCGAGCACTAAGAGAACCTTTGG | 105 |
LAPTM5 | CATCTTTTCCATCGCCTTCATCAC | TCCACCGAGTTCATGCACTTG | 102 |
MAPKAPK3 | TCCCACCCTTCTACTCCAACA | TTCAACAGGAGGCGGATCA | 141 |
NR3C1 | AGAGGAGGAGCTACTGTGAAGG | ACTGAGCCTTTTGGAAAATCAACC | 109 |
PAX2 | CCCAGAGTGGTGTGGACAGTTT | GTAGGAAGGACGCTCAAAGACC | 101 |
PSAT1 | ACGCCTCCATGTTTCAGCAT | TGAGATTTGATGGAGCTAAGCTTCT | 104 |
PTCH1 | GTCGAGCTGTTCGGCATGAT | AGCAACGTGAACGGTGAACTC | 111 |
RAB25 | GGAGCTCTATGACCATGCTGAA | CCAGGAAGAGCAGTCCATTGTT | 125 |
RXRA | AGGACTGCCTGATTGACAAGC | GACTCCACCTCATTCTCGTTCC | 141 |
SLPI | GCTGTGGAAGGCTCTGGAAA | TGCCCATGCAACACTTCAAG | 298 |