Skip to main content

Table 2 Oligonucleotide primers used for the quantitative real time PCR.

From: TLR3 and TLR4 expression in healthy and diseased human endometrium

Gene (GenBank No.) Forward primers (position) Reverse primers (position)
TLR3 (NM_003265) 5'-GTATTGCCTGGTTTGTTAATTGG (2059–2082) 5'-AAGAGTTCAAAGGGGGCACT (2215–2194)
TLR4 (NM_138557) 5'-AAGCCGAAAGGTGATTGTTG (2187–2206) 5'-CTGAGCAGGGTCTTCTCCAC (2339–2320)
ACTB (NM_001101) 5'-ACCAACTGGGACGACATGGA (302–322) 5'-CCAGAGGCGTACAGGGATAG (510–491)
  1. All primers were designed using the Primer3 software.