Table 2 Oligonucleotide primers used for the quantitative real time PCR.
From: TLR3 and TLR4 expression in healthy and diseased human endometrium
Gene (GenBank No.) | Forward primers (position) | Reverse primers (position) |
|---|---|---|
TLR3 (NM_003265) | 5'-GTATTGCCTGGTTTGTTAATTGG (2059–2082) | 5'-AAGAGTTCAAAGGGGGCACT (2215–2194) |
TLR4 (NM_138557) | 5'-AAGCCGAAAGGTGATTGTTG (2187–2206) | 5'-CTGAGCAGGGTCTTCTCCAC (2339–2320) |
ACTB (NM_001101) | 5'-ACCAACTGGGACGACATGGA (302–322) | 5'-CCAGAGGCGTACAGGGATAG (510–491) |