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Table 3 Secondary anti-bodies used in the study, their dilution, buffers and incubation time.

From: Impaired leukocyte influx in cervix of postterm women not responding to prostaglandin priming

Protein 2nd Ab Dilution Buffer Incubation
CD45RB biotinylated horse
anti-mouse
1:200 1.5% NHS in PBS 60 min RT
CD68 biotinylated horse
anti-mouse
1:200 1.5% NHS in PBS 60 min RT
MMP-9 biotinylated horse
anti-mouse
1:200 1.5% NHS in PBS 60 min RT
IL-8 biotinylated horse
anti-mouse
1:200 1.5% NHS in PBS 30 minRT
PAF-R biotinylated horse
anti-mouse
1:200 1.5% NHS in TBS 30 min RT
S-1
(CD138)
biotinylated horse
anti-mouse
1:200 1.5% NHS in PBS 30 min RT
ERα biotinylated horse
anti-mouse
1:200 1.5% NHS in PBS 30 minRT
ERβ biotinylated horse
anti-mouse
1:200 1.5% NHS in TBS + 5% BSA 60 min RT
GPR30 biotinylated goat
anti-rabbit
1:200 1.5% NGS in PBS + 5% BSA 30 min RT
Ki67 biotinylated horse
anti-mouse
1:200 1.5% NHS in PBS 30 min RT
  1. biotinylated horse anti-mouse antibody (Vector, Cat# BA-2000).
  2. biotinylated goat anti-rabbit antibody (Vector, Cat# BA-1000).