Table 3 Secondary anti-bodies used in the study, their dilution, buffers and incubation time.
From: Impaired leukocyte influx in cervix of postterm women not responding to prostaglandin priming
Protein | 2nd Ab | Dilution | Buffer | Incubation |
|---|---|---|---|---|
CD45RB | biotinylated horse anti-mouse | 1:200 | 1.5% NHS in PBS | 60 min RT |
CD68 | biotinylated horse anti-mouse | 1:200 | 1.5% NHS in PBS | 60 min RT |
MMP-9 | biotinylated horse anti-mouse | 1:200 | 1.5% NHS in PBS | 60 min RT |
IL-8 | biotinylated horse anti-mouse | 1:200 | 1.5% NHS in PBS | 30 minRT |
PAF-R | biotinylated horse anti-mouse | 1:200 | 1.5% NHS in TBS | 30 min RT |
S-1 (CD138) | biotinylated horse anti-mouse | 1:200 | 1.5% NHS in PBS | 30 min RT |
ERα | biotinylated horse anti-mouse | 1:200 | 1.5% NHS in PBS | 30 minRT |
ERβ | biotinylated horse anti-mouse | 1:200 | 1.5% NHS in TBS + 5% BSA | 60 min RT |
GPR30 | biotinylated goat anti-rabbit | 1:200 | 1.5% NGS in PBS + 5% BSA | 30 min RT |
Ki67 | biotinylated horse anti-mouse | 1:200 | 1.5% NHS in PBS | 30 min RT |