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Table 2 Antibodies used in the study.

From: Impaired leukocyte influx in cervix of postterm women not responding to prostaglandin priming

Protein Order number and company Type Dilution Incub. time Blocking
CD45RB M0833 (B cells, T cells subsets, monocytes, macrophages and some granulocytes) DAKO A/S. Mc mouse
anti-human
1:200 +4°C o/n D
CD68 M0876 (macrophages) DAKO A/S, Glostrup, Denmark Mc mouse
anti-human
1:200 +4°C o/n D
MMP-9 MS-817-P0, NeoMarkers Inc Mc mouse
anti-human
1:400 RT 60 min D
IL-8 sc-73321, Santa Cruz Biotechnology, Inc Mc mouse
anti-human
1:200 +4°C o/n A
PAF-R 160600, Cayman chemical Mc mouse
anti-human
1:350 +4°C o/n E
S-1
(CD138)
M7228, DakoCytomation Mc mouse
anti human
1:50 RT
30 min
A
ERα 2nd Gen 08-1149, Zymed Laboratories Inc Mc mouse
anti-human
1:5 +4°C o/n A
ERβ Serotec Ltd, MCA1974 Mc mouse
anti-human
1:20 +4°C o/n B
GPR30 LS-A4272, LifeSpan BioSciences Pc rabbit
anti-human
1:300 RT
60 min
C
Ki-67 NCL-Ki67-MM1, Novocastra Laboratories Ltd Mc mouse
anti human
1:200 +4°C o/n A
  1. Mc = monoclonal; Pc = polyclonal, A = 1.5% normal horse serum (NHS) in PBS for 30 min, B = 1.5% normal horse serum (NHS) in TBS containing 5% bovine serum albumin (BSA) for 45 min, C = 1.5% normal goat serum (NGS) in PBS containing 5% bovine serum albumin (BSA) for 30 min, D = 1.5% normal horse serum (NHS) in PBS for 45 min, E = 1.5% normal horse serum (NHS) in TBS for 45 min.
  2. NHS = normal horse serum (Vector Laboratories, Burlingame, CA).
  3. NGS = normal goat serum (DakoCytomation, Glostrup, Denmark).