Figure 3

Comparative results from flow cytometry analysis of EGFP-expression in seminiferous epithelium cells transfected in vivo with pRed or pGtoR. (A) Representative traces of flow cytometry using pGtoR vector. 1) R2 corresponds to non-transfected cell population; R3 represent the transfected cells (red fluorescent). 2) M1 represents the level of green fluorescent (EGFP) of R2 cell population 3) represents the level of EGFP in transfected cells (R3). B) The histogram shows the mean (Mean ± SE) intensity of green fluorescence of the cells (EGFP) transfected in vivo with pRed or pGtoR, as determined by flow cytometry four days after transfections. Asterisk shows statistically significant differences as measured by p < 0.05.