Figure 2

Seminiferous tubules of testis from C57BL/6J wild type mice after in vivo transfection with the pEGFP-N1 vector. A and C) Merge image of partial view of tubule sections showing EGFP-positive Sertoli cells (with the well-known arborescent-like cytoplasm) as preferentially transfected cells. Nuclei were stained with Hoechst 33258 dye. Spermatocytes (Sp) round spermatid (Rd) are indicated (C). The red lines indicate the basement membranes of seminiferous tubules. B and D show enlarged images of EGFP fluorescence taken 24 hours after transfection. Bars represent 50 μm.