Western blot analysis of chorionic villi of the placentae from normal and selected abnormal pregnancies with antibody TRO-tf-01. Actin is used as a control for protein loading; lysates (NP-40) were loaded on 10% acrylamide gel (SDS, reducing conditions). Quantitative evaluation of TRO-tf-01 expression (O.D.) in the placentae is placed at the bottom. Placentae of mothers with 1 – drug abuse during pregnancy (DrA), 2 – pregnancy-induced hypertension together with gestational diabetes (PIH+GD), 3 – gestational diabetes (GD), 4 – normal pregnancy, 5 – pregnancy-induced hypertension (PIH).