Placenta lysate stained with CBB after 2D-SDS PAGE (A) and immunodetection with TRO-tf-01 antibody (B). A 13 cm Immobiline DryStrip of pH range 4–7 was used for the separation of the total protein lysate according to pI, in the second dimension, 10% polyacrylamide slab gel (SDS) was used. After immunoblotting with MoAb TRO-tf-01, only one isoform (arrow) of transferrins (underlined) was labeled (B). Detected antigen was identified by MALDI-TOF analysis as human transferrin (TF, sequence coverage of 31%, Z-score of 2.31). Molecular weight is shown on the left.