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Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study

Figure 1

Placenta lysate stained with CBB after 2D-SDS PAGE (A) and immunodetection with TRO-tf-01 antibody (B). A 13 cm Immobiline DryStrip of pH range 4–7 was used for the separation of the total protein lysate according to pI, in the second dimension, 10% polyacrylamide slab gel (SDS) was used. After immunoblotting with MoAb TRO-tf-01, only one isoform (arrow) of transferrins (underlined) was labeled (B). Detected antigen was identified by MALDI-TOF analysis as human transferrin (TF, sequence coverage of 31%, Z-score of 2.31). Molecular weight is shown on the left.

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