Figure 1

Alternative splicing of the HTRA3 gene and strategy to analyze the two transcripts by RT-PCR. A schematic illustration of the alternative splicing of the HTRA3 gene to show how the long and the short transcripts are generated and analyzed. ATG, start codon; TGA, stop codon. Fragment HTRA3-L, amplified by HTRA3-L F (forward) and HTRA3-L R (reverse) primers listed in Table 1, across exon 9 and 10, represents the long specific transcript; fragment HTRA3-S, amplified by HTRA3-S F (forward) and HTRA3-S R (reverse) primers listed in Table 1, located on exon 7, represents the short specific transcript.