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Table 3 Analysis of total and messenger RNA yields and transcript levels across different stage ovaries of coho salmon (N = 5 fish/stage) in August 2006.

From: Identification of differentially expressed ovarian genes during primary and early secondary oocyte growth in coho salmon, Oncorhynchus kisutch

Parameter Cohort 1 Cohort 2 Cohort 1: mRNA based Cohort 2: mRNA based Fold change (mRNA) Cohort 1: total RNA based Cohort 2: total RNA based Fold change (total RNA)
Body mass (g) 77.1 ± 9.5 736.5 ± 33.8       
Fork length (cm) 17.7 ± 0.7 36.4 ± 0.9       
Paired ovary mass (g) 0.22 ± 0.02 8.51 ± 0.71       
Gonadosomatic Index (GSI) 0.30 ± 0.03 1.15 ± 0.05       
Follicle stage late perinucleolus yolk granule       
Est. fecundity (no. of follicles) 3361 ± 192 2999 ± 462       
μg total RNA/g ovary 4750 ± 80 1509 ± 40       
μg total RNA/follicle 0.31 ± 0.01 4.50 ± 0.50       
ng mRNA/μg total RNA 117.8 ± 4.0 23.4 ± 3.2       
ng mRNA/follicle 36.4 ± 1.6 99.5 ± 4.7       
% mRNA 11.8 ± 0.4 2.3 ± 0.3       
Housekeeping gene         
ef1a per template RNA    1.02 ± 0.01 1.18 ± 0.03 up 1.2 1.94 ± 0.05 1.33 ± 0.09 down 1.5
ef1a per follicle    1.14 ± 0.04 1.16 ± 0.04 nc 5.61 ± 0.15 1.24 ± 0.08 down 4.5
Downregulated gene         
fth3 per template RNA    4.25 ± 0.20 1.35 ± 0.13 down 3.2 18.60 ± 0.46 1.69 ± 0.26 down 11.0
fth3 per follicle    4.89 ± 0.20 1.38 ± 0.16 down 3.5 58.27 ± 1.99 1.69 ± 0.22 down 34.5
fth3/ef1a    4.69 ± 0.21 1.29 ± 0.13 down 3.6 9.81 ± 0.25 1.29 ± 0.19 down 7.6
Upregulated gene         
fshr per template RNA    1.58 ± 0.35 12.48 ± 2.53 up 7.9 1.16 ± 0.05 3.44 ± 0.38 up 3.0
fshr per follicle    1.82 ± 0.43 12.57 ± 2.70 up 6.9 1.22 ± 0.06 1.17 ± 0.12 nc
fshr/ef1a    1.57 ± 0.35 10.81 ± 2.41 up 6.9 1.23 ± 0.08 5.25 ± 0.34 up 4.3
  1. Cohort 1 fish were brood year 2004 and cohort 2 fish were brood year 2003.
  2. nc, no change