Identification of differentially expressed ovarian genes during primary and early secondary oocyte growth in coho salmon, Oncorhynchus kisutch

Table 3 Analysis of total and messenger RNA yields and transcript levels across different stage ovaries of coho salmon (N = 5 fish/stage) in August 2006.

Parameter	Cohort 1	Cohort 2	Cohort 1: mRNA based	Cohort 2: mRNA based	Fold change (mRNA)	Cohort 1: total RNA based	Cohort 2: total RNA based	Fold change (total RNA)
Body mass (g)	77.1 ± 9.5	736.5 ± 33.8
Fork length (cm)	17.7 ± 0.7	36.4 ± 0.9
Paired ovary mass (g)	0.22 ± 0.02	8.51 ± 0.71
Gonadosomatic Index (GSI)	0.30 ± 0.03	1.15 ± 0.05
Follicle stage	late perinucleolus	yolk granule
Est. fecundity (no. of follicles)	3361 ± 192	2999 ± 462
μg total RNA/g ovary	4750 ± 80	1509 ± 40
μg total RNA/follicle	0.31 ± 0.01	4.50 ± 0.50
ng mRNA/μg total RNA	117.8 ± 4.0	23.4 ± 3.2
ng mRNA/follicle	36.4 ± 1.6	99.5 ± 4.7
% mRNA	11.8 ± 0.4	2.3 ± 0.3
Housekeeping gene
ef1a per template RNA			1.02 ± 0.01	1.18 ± 0.03	up 1.2	1.94 ± 0.05	1.33 ± 0.09	down 1.5
ef1a per follicle			1.14 ± 0.04	1.16 ± 0.04	nc	5.61 ± 0.15	1.24 ± 0.08	down 4.5
Downregulated gene
fth3 per template RNA			4.25 ± 0.20	1.35 ± 0.13	down 3.2	18.60 ± 0.46	1.69 ± 0.26	down 11.0
fth3 per follicle			4.89 ± 0.20	1.38 ± 0.16	down 3.5	58.27 ± 1.99	1.69 ± 0.22	down 34.5
fth3/ef1a			4.69 ± 0.21	1.29 ± 0.13	down 3.6	9.81 ± 0.25	1.29 ± 0.19	down 7.6
Upregulated gene
fshr per template RNA			1.58 ± 0.35	12.48 ± 2.53	up 7.9	1.16 ± 0.05	3.44 ± 0.38	up 3.0
fshr per follicle			1.82 ± 0.43	12.57 ± 2.70	up 6.9	1.22 ± 0.06	1.17 ± 0.12	nc
fshr/ef1a			1.57 ± 0.35	10.81 ± 2.41	up 6.9	1.23 ± 0.08	5.25 ± 0.34	up 4.3

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