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Table 2 Selected salmon ovarian genes revealed by SSH and further assessed with qPCR.

From: Identification of differentially expressed ovarian genes during primary and early secondary oocyte growth in coho salmon, Oncorhynchus kisutch

Putative gene identity EST Size of query (bp) E-value Species Accession no. GO molecular function term(s)
Factor in the germline alpha (figla) EX152185 257 9E-14 K. marmoratus ABG89136 GO:0030528 transcription regulator activity
Zona pellucida glycoprotein C (zpc) EX152517 277 8E-09 D. rerio AAI16532  
Zona pellucida protein X (zpx) EX152510 429 9E-27 S. aurata AAY21008  
Egg envelope glycoprotein (eeg) EX152158 592 6E-34 D. rerio XP_001335392  
Somatic lipoprotein receptor (vldlro) EX152595 546 8E-14 O. mykiss CAA05874 GO:0004872 receptor activity
       GO:0005509 calcium ion binding
Cathepsin B, a (ctsba) EX152225 495 4E-29 D. rerio NP_998501 GO:0004197 cysteine-type endopeptidase activity
Cathepsin D (ctsd) EX152680 327 1E-123 * O. mykiss U90321 GO:0004190 aspartic-type endopeptidase activity
       GO:0004192 cathepsin D activity
       GO:0004194 pepsin A activity
       GO:0016787 hydrolase activity
Cathepsin Z (ctsz)† EX152609 624 5E-172 * O. mykiss AAT94061 GO:0004197 cysteine-type endopeptidase activity
       GO:0008234 cysteine-type peptidase activity
Serum lectin isoform 2 (lcal) EX152541 513 1E-34 S. salar AAO43606 GO:0005529 sugar binding
Rhamnose binding lectin STL3 (lrham) EX152294 324 8E-52 O. mykiss BAA92257 GO:0005529 sugar binding
       GO:0016524 latrotoxin receptor activity
Alveolin (alv) EX152194 532 4E-36 O. latipes BAA90750 GO:0008237 metallopeptidase activity
       GO:0008533 astacin activity
Cyclin E (ccne) EX152152 800 3E-53 D. rerio NP_571070 GO:0016301 kinase activity
Ferritin H-3 (fth3) EX152267 552 2E-91 O. mykiss BAA13148 GO:0005488 binding
       GO:0005506 iron ion binding
       GO:0008199 ferric iron binding
       GO:0016491 oxidoreductase activity
       GO:0046872 metal ion binding
       GO:0046914 transition metal ion binding
DnaJ subfamily A member 2 (dnaja2) EX152440 334 2E-55 D. rerio NP_998658 GO:0031072 heat shock protein binding
       GO:0051082 unfolded protein binding
Coatomer protein epsilon subunit (cope)†† EX152380 438 6E-44 O. mykiss AAM18476  
Anterior pharynx defective 1b (aph1b) EX152190 601 6E-90 D. rerio NP_956409 GO:0005515 protein binding
Retinol dehydrogenase 1 (rdh1) EX152534 565 7E-71 O. latipes ABQ09278 GO:0016491 oxidoreductase activity
  1. † The top blast hit was cathepsin Y, which is a synonym for cathepsin Z used here.
  2. †† The top blast hit was named VHSV induced protein, but this appears to be a coatomer protein subunit based on similarity to other sequences.
  3. * The blastn similarity score is shown instead of blastx due to the sequence corresponding primarily or completely to the 3' UTR.
  4. Complementary DNA sequences for additional candidate genes were obtained from a follicle/interstitial cell enriched library [29].