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Table 2 Selected salmon ovarian genes revealed by SSH and further assessed with qPCR.

From: Identification of differentially expressed ovarian genes during primary and early secondary oocyte growth in coho salmon, Oncorhynchus kisutch

Putative gene identity

EST

Size of query (bp)

E-value

Species

Accession no.

GO molecular function term(s)

Factor in the germline alpha (figla)

EX152185

257

9E-14

K. marmoratus

ABG89136

GO:0030528 transcription regulator activity

Zona pellucida glycoprotein C (zpc)

EX152517

277

8E-09

D. rerio

AAI16532

 

Zona pellucida protein X (zpx)

EX152510

429

9E-27

S. aurata

AAY21008

 

Egg envelope glycoprotein (eeg)

EX152158

592

6E-34

D. rerio

XP_001335392

 

Somatic lipoprotein receptor (vldlro)

EX152595

546

8E-14

O. mykiss

CAA05874

GO:0004872 receptor activity

      

GO:0005509 calcium ion binding

Cathepsin B, a (ctsba)

EX152225

495

4E-29

D. rerio

NP_998501

GO:0004197 cysteine-type endopeptidase activity

Cathepsin D (ctsd)

EX152680

327

1E-123 *

O. mykiss

U90321

GO:0004190 aspartic-type endopeptidase activity

      

GO:0004192 cathepsin D activity

      

GO:0004194 pepsin A activity

      

GO:0016787 hydrolase activity

Cathepsin Z (ctsz)†

EX152609

624

5E-172 *

O. mykiss

AAT94061

GO:0004197 cysteine-type endopeptidase activity

      

GO:0008234 cysteine-type peptidase activity

Serum lectin isoform 2 (lcal)

EX152541

513

1E-34

S. salar

AAO43606

GO:0005529 sugar binding

Rhamnose binding lectin STL3 (lrham)

EX152294

324

8E-52

O. mykiss

BAA92257

GO:0005529 sugar binding

      

GO:0016524 latrotoxin receptor activity

Alveolin (alv)

EX152194

532

4E-36

O. latipes

BAA90750

GO:0008237 metallopeptidase activity

      

GO:0008533 astacin activity

Cyclin E (ccne)

EX152152

800

3E-53

D. rerio

NP_571070

GO:0016301 kinase activity

Ferritin H-3 (fth3)

EX152267

552

2E-91

O. mykiss

BAA13148

GO:0005488 binding

      

GO:0005506 iron ion binding

      

GO:0008199 ferric iron binding

      

GO:0016491 oxidoreductase activity

      

GO:0046872 metal ion binding

      

GO:0046914 transition metal ion binding

DnaJ subfamily A member 2 (dnaja2)

EX152440

334

2E-55

D. rerio

NP_998658

GO:0031072 heat shock protein binding

      

GO:0051082 unfolded protein binding

Coatomer protein epsilon subunit (cope)††

EX152380

438

6E-44

O. mykiss

AAM18476

 

Anterior pharynx defective 1b (aph1b)

EX152190

601

6E-90

D. rerio

NP_956409

GO:0005515 protein binding

Retinol dehydrogenase 1 (rdh1)

EX152534

565

7E-71

O. latipes

ABQ09278

GO:0016491 oxidoreductase activity

  1. † The top blast hit was cathepsin Y, which is a synonym for cathepsin Z used here.
  2. †† The top blast hit was named VHSV induced protein, but this appears to be a coatomer protein subunit based on similarity to other sequences.
  3. * The blastn similarity score is shown instead of blastx due to the sequence corresponding primarily or completely to the 3' UTR.
  4. Complementary DNA sequences for additional candidate genes were obtained from a follicle/interstitial cell enriched library [29].