Figure 2

Influence of ActA and InA on the F-actin cytoskeleton of DCs. iDCs generated from PBMCs of healthy donors were differentiated into mDCs in the presence or absence of Dex, TGF-β1, ActA and InA. Changes in cell morphology were assessed by confocal microscopy following staining with Phalloidin-Alexa488 to visualize F-Actin structures. (A) Representative pictures of iDCs, mDCs and DCs matured in the presence of one of the four hormones are depicted; bar: 20 μm. (B) Quantification of the number of podosome-forming cells for each experimental condition as a measure of the degree of DC maturation. For each analysis 100–200 DCs were randomly counted. Mean +/- SEM; n = 5; ** p < 0.01, * p < 0.05 (hormonal treatment vs. mDC).