Figure 5

Representative micrograph of staining for factor VIII (black color) in ovarian tissues from autotransplanted (A, B, C, D; left column) and control (E, F, G, H; right column) ewes. Note the presence of factor VIII in blood vessels in the area containing primordial/primary (A, C) and antral follicles (B, F), in the theca layer of preovulatory follicles (C, G), and in capillaries of the CL (D, H). Arrows indicate primordial/primary follicles. G = granulosa and T = theca in preovulatory follicles in B, C, F and G. Control sections did not exhibit any positive staining (see Fig. 8 insert). Bar = 50 μm for A, C, D, E, G, H and 100 μm for B and F.