Figure 7

Effect of STZ treatment on the phosphorylation levels of AMPK and MAPK ERK1/2 in rat ovary (A), muscle (B) and liver (C). Lysates (50 μg) of ovary, muscle and liver from control and STZ-treated rats were resolved by SDS-PAGE, transferred to nitrocellulose membrane, and probed with anti-phospho-AMPK, phospho-MAPK ERK1/2 and then with anti-AMPKα1 and anti-ERK2 antibodies, respectively. Blots were quantified and the phosphorylated protein/total protein ratio is shown. Data are shown as means ± SE, with n = 6 for the control group and n = 12 for the STZ-treated group. *p < 0.05, *p < 0.001.