Figure 6
Effect of STZ treatment on the plasma levels of adiponectin and resistin (A) and on the protein levels of AdipoR1 and AdipoR2 in rat ovary (B), muscle (C) and liver (D). A.A. Plasma adiponectin and resistin concentrations were determined by ELISA. C: control; STZ: streptozotocin. *p < 0.05, ***p < 0.001. B, C and D. Lysates (50 μg) of ovary (B), muscle (C) and liver (D) from control and STZ-treated rats were resolved by SDS-PAGE, transferred to nitrocellulose membrane, and probed with anti-AdipoR1 or anti-AdipoR2 antibodies. Equal protein loading was verified by reprobing the membrane with an anti-Vinculin antibody. Blots were quantified and the AdipoR1, AdipoR2/vinculin ratios are shown. Data are shown as means ± SE, with n = 6 for the control group and n = 12 for the STZ-treated group. *p < 0.05, *p < 0.001.