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Figure 5 | Reproductive Biology and Endocrinology

Figure 5

From: Effects of high levels of glucose on the steroidogenesis and the expression of adiponectin receptors in rat ovarian cells

Figure 5

Effect of STZ treatment on the amounts of the 3βHSD, p450scc, StAR and p450 aromatase proteins in rat ovarian cells. Protein extracts of ovaries from control and STZ-treated rats were subjected to SDS-PAGE as described in Materials and Methods. The membranes were incubated with antibodies raised against the 3βHSD (A), p450scc (B), StAR (C) and p450 aromatase (D) proteins. Equal protein loading was verified by reprobing membrane with an anti-Vinculin antibody. Blots were quantified and the 3βHSD, p450scc, StAR and p450 aromatase/Vinculin ratios are shown. Data are shown as means ± SE, with n = 6 for the control group and n = 12 for the STZ-treated group. *p < 0.05, *p < 0.001.

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