Figure 3

Effect of glucose treatment on MAPK ERK1/2 phosphorylation in rat granulosa cells. Granulosa cell lysates were prepared from cells incubated with 10 g/l glucose for various times: 0, 5, 10, 30, 60 or 120 min (A) or with 10 g/l glucose for 48 h in the presence or absence of FSH (10^-8M) or IGF-1 (10^-8M). Lysates (50 μg) were resolved by SDS-PAGE, transferred to nitrocellulose membrane, and probed with anti-phospho-MAPK ERK1/2 and then with anti-ERK2 antibodies. Representative blots from three independent experiments are shown. Blots were quantified and the phosphorylated MAPK ERK1/2/ERK2 protein ratio is shown. The results are represented as means ± SE. Bars with different letters are significantly different (p < 0.05). The letter "a" indicates values which are not significantly different from control (without FSH or IGF-1 and glucose).