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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: Effects of high levels of glucose on the steroidogenesis and the expression of adiponectin receptors in rat ovarian cells

Figure 2

Effect of glucose treatment on the amounts of the 3βHSD, p450scc, StAR and p450 aromatase proteins in rat granulosa cells. Protein extracts from rat granulosa cells cultured for 48 h in the absence or in the presence of 10 g/l glucose ± 10-8 M FSH or 10-8 M IGF-1 were subjected to SDS-PAGE as described in Materials and Methods. The membranes were incubated with antibodies raised against the 3βHSD (A), p450scc (B), StAR (C) and p450 aromatase (D) proteins. Equal protein loading was verified by reprobing membranes with an anti-vinculin antibody. Results are representative of at least three independent experiments. Blots were quantified and the 3βHSD, p450scc, StAR and p450 aromatase to Vinculin ratios are shown. The results are expressed as means ± SE. Bars with different letters are significantly different (p < 0.05). The letter "a" indicates values which are not significantly different from control (without FSH or IGF-1 and glucose).

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