Immunolocalization of RELMα in a untreated 180-day old LY mouse ovary. VectaStain Rabbit IgG Elite ABC kit (Vector Laboratories, Inc., Burlingame, CA) was used on paraffin sections. Sections were incubated with primary antibody (anti-RELMα; Upstate/Chemicon, Temecula, CA) for 1 h at RT, washed thrice, and incubated with biotinylated second antibody for 30 min. at RT. DAB substrate (brown precipitate, arrows) was used for antigen detection, and sections were counterstained with hematoxylin. Scale bar = 25 μm (lower right).