Table 1 .
From: Expression of costimulatory molecules in the bovine corpus luteum
Target | Primer Sequence | Amplicon Size | Annealing Temp (°C) | GenBank Accession no. | Reference |
|---|---|---|---|---|---|
CD80 | Forward: 5'-GAACCGCACCATCACTGACA-3' Reverse: 5'-TAATGGTCCAGGTCAGGTGC-3' | 485 bp | 56°C | Y09950 | [40] |
CD86 | Forward: 5'-GACCTTGAGACTCCACAACG-3' Reverse: 5'-GTAGAGCTGCAATCCAGAGG-3' | 490 bp | 58°C | AJ291475 | - |
CD31 | Forward: 5'-GTTCAGCGAAGTTCTGCGAG-3' Reverse: 5'-CTTGCTGGCTGTGGTCTTGT-3' | 229 bp | 58°C | U35433 | [43] |
GAPDH | Forward: 5'-AAGATTGTCAGCAATGCC-3' Reverse: 5'-ACAGACACGTTGGGAG-3' | 293 bp | 56°C | BC102589 | - |
GAPDH | Forward: 5'-TGTTCCAGTATGATTCCACCC-3' Reverse: 5'-TCCACCACCCTGTTGCTGTA-3' | 854 bp | 58°C | NM_001034034 | [41] |
StAR | Forward: 5'-CCTCTCTACAGCGACCAA-3' Reverse: 5'-TCGTGAGTGATGACCGTG-3' | 311 bp | 58°C | Y17259 | [42] |