Figure 2

SP-D immunostaining in mouse male reproductive tissues. Photographs in the left column are tissues immunostained for SP-D. Corresponding photographs on the right are the same tissues immunostained using normal rabbit IgG instead of the primary antibody. A and B. Testis. The interstitial cells (asterisks) of the testis stained positively for SP-D protein. Sertoli cells and spermatids also stained positively (arrows). C and D. Epididymis. SP-D immunostaining was observed in the epithelium of the epididymis (arrows). E and F. Seminal vesicle. Almost no SP-D immunostaining was observed in the seminal vesicle. Secreted material in the lumen of the gland was stained non-specifically in both the immunostained sections and in the normal IgG control sections. G and H. Prostate. SP-D immunostaining was present in the epithelium of the prostate (arrows). Reproductive tissues obtained from at least three different mice were immunostained with similar results. Magnification bar = 100 μm.