Figure 5

Measurements of intracellular Ca²⁺ concentrations in human GCs. A) Line plot graphic showing the time-course of intracellular Ca²⁺ changes for three representative cells sequentially treated with buffer (at t = 0), 0.1 μM DA, and 100 μM carbachol. Color images of fluo-4 AM loaded GCs of identical viewing areas are also provided. Color changes from red/yellow to yellow/blue represent increased intracellular Ca²⁺ levels. B) Line plot graphic showing the time-course of intracellular Ca²⁺ changes for three representative cells sequentially treated with buffer (t = 0), 1 nM L-741,626, 0.1 μM DA, and 100 μM carbachol, as a positive control. Data are expressed as relative fluorescence intensities corresponding to a pseudo color scale from red/yellow to yellow/blue. Experiments were repeated seven times with a total of 60 to 90 cells each examined in detail. C) Percentage of GCs responding to DA with increased intracellular Ca²⁺ levels. The graphic represents the percentage of GCs (day 3 or 4 of culture) responding to DA (0.1 μM) with an increase in the intracellular Ca²⁺ levels. GCs were either untreated or stimulated with hCG (10 IU/ml) since the 1^st day of culture. Experiments were repeated seven times with a total of 60 to 90 cells each examined in detail. Data represent means + SEM (ANOVA/post-test *p < 0.05).