Invasion assay and expression of MMPs and TIMPs mRNA in EN-1078D. A) EN-1078D cells showed capacities to increase their invasiveness by 2 fold induction when they were stimulated with TGF-β3. B) The cell line expresses MMP-2 and 14 while MMP-9 was not detected. The signal for MMP-2 is stronger for EN-1078D cells than for the positive control used. C) Both TIMPs expressions were detected but TIMP-2 signal is weak. Data shown are representative of results obtained from three independent assays.