A) Karyotype analysis of EN-1078D (P76+13 passages), showing that all cells have monosomy of X chromosome. Most of the cells have trisomy of chromosome 17. B) FISH analysis of copy number of X chromosome with Probe CEP X (DXZ1) alpha satellite III. Probe DXZ1 marked in orange spectrum hybrid satellite DNA localized in the centromere of the X chromosome. Only one X chromosome per cell was observed. C) Metaphasic analysis of Her2/neu (human epidermal growth factor receptor 2) by fluorescence in situ hybridization (FISH). The HER-2 probe spans the entire HER-2 gene is labeled in spectrum orange. The CEP 17 probe is labeled in spectrum green and hybridizes to the alpha satellite DNA located at the centromere of chromosome 17 (17p11.1-q11.1). Inclusion of the CEP 17 probe allows for the relative copy number of the HER-2 gene to be determined.