Expression of cytokeratins in EN-1078D. A) RT-PCR analysis for stratified-, simple-epithelial cytokeratins and for the other types of intermediate filaments. In EN-1078D cells, the simple-epithelial cytokeratins are predominantly expressed. Vimentin expression is consistent with the neoplasic tissue while absence of the expression of desmin confirmed that EN-1078D is not an endometrial sarcoma. B) Expression of cytokeratins 18 and 19 by Western Blot analysis KLE was used like as positive control Data shown are representative of results obtained from three independent assays.