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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: PKCepsilon and an increase in intracellular calcium concentration are necessary for PGF2alpha to inhibit LH-stimulated progesterone secretion in cultured bovine steroidogenic luteal cells

Figure 4

Effects of PKCε down-regulation on the ability of PGF2α to inhibit the LH-stimulated progesterone synthesis/secretion in cultures of steroidogenic luteal cells transfected for 96 h with PKCε specific siRNA (filled bars) or with transfection regents (control, open bars). Progesterone accumulation was determined in culture media after 4 h of incubation in the following treatments: LH (100 ng/ml), PGF2α (1 μg/ml) and a combination of PGF2α and LH. Data are presented as mean ± SEM of four individual replicates (n = 4 cows). For each treatment group, statistical comparisons were made between PKCε down-regulated (PKCε siRNA) and control (not PKCε down-regulated); different letters above each SEM denote different values, P < 0.05.

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