Figure 3

PKCα and PKCβII protein after 96 h transfection of luteal steroidogenic cells with PKCε specific siRNA. (A) Representative Western blot showing the amount of PKCα, PKCβII KCε and actin detected in protein samples prepared from luteal steroidogenic cells after 96 h of transfection with PKCε specific siRNA (lane 1). Lanes labeled 2 – 4, contained protein samples from indicated control treatments (media, Non-sp siRNA, and transfection reagent, respectively. B and C) Semi-quantitative analysis of the densitometry derived from four experiments similar to the one shown in panel A for PKCα (B) and PKCβII (C). The y-axis shows the ratio of the optical density ratio of PKC isozyme to that of its corresponding β-actin. The data are shown as mean ± SEM, and comparisons were made between different treatments by one-way ANOVA followed by Tukey-Kramer honestly significant difference.