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Table 3 List of protein spots and their contents identified by MS/MS in leiomyomas and myometrium

From: Genomic and proteomic profiling I: Leiomyomas in African Americans and Caucasians

Protein Spots

Description

Accession Number

Theoretical MW

Theoretical pI

Score

Match

Transcripts *

76

Annexin A1

P04083

38.6

6.64

361

10

+

 

Aflatoxin B1 Aldehyde

reductase 2

O43488

39.6

6.70

359

11

+

 

Splice Isoform 1

P31942

36.9

6.37

327

9

+

 

Heterogeneous nuclear ribonucleoprotein H3

      
 

PDZ and LIM domain protein 1

O00151

35.9

6.55

257

5

+

 

PTD012 protein

Q6FI88

35.1

6.23

217

7

+

 

LIM and SH3 domain protein 1

Q14847

29.7

6.61

114

3

+

93

CGI-150 protein

Q9Y3E8

55.0

8.89

495

13

+

 

Inorganic pyrophosphatase

Q9H2U2

38.0

7.07

106

3

+

107

Annexin V

P08758

35.8

4.94

619

23

+

 

Mimecan

P20774

33.9

5.46

329

6

+

 

Microtubule-associated protein RP/EB1

Q15691

29.9

5.02

180

4

+

 

EF-hand domain-containing protein 2

Q96C19

26.7

5.15

155

3

+

 

Tubulin-specific chaperone B

Q99426

27.3

5.06

132

3

+

126

14-3-3ε

Q04917

28.1

4.76

339

8

+

 

14-3-3Ï„

P27348

27.8

4.68

317

7

+

 

14-3-3γ

P61981

28.2

4.80

231

8

+

 

14-3-3β/α

P31946

28.0

4.76

170

6

+

 

Annexin V

P08758

35.8

4.94

162

4

+

 

Proteasome α5

P28066

26.4

4.74

157

4

 
 

Vimentin

P08670

53.5

5.06

124

3

+

 

Acidic leucine-rich nuclear phosphoprotein 32A

P39687

28.6

3.99

92

2

+

183

α crystallin B chain

P02511

20.2

6.76

489

28

+

187

Transgelin (SM22)

Q5U0D2

22.6

8.87

632

28

+

192

Myosin regulatory light chain 2, smooth muscle isoforms

P24844

19.7

4.8

333

15

+

 

Myosin regulatory light chain 2, nonsarcomeric

P19105

19.7

4.67

224

9

+

204

Splice Isoform CFL2b of

Q9Y281

18.7

7.66

263

10

+

 

Cofilin, muscle isoforms

     

+

 

Cofilin, non-muscle isoforms

P23528

18.4

8.26

172

6

+

 

Transgelin (SM22)

Q01995

22.5

8.88

96

3

+

206

Transgelin (SM22)

Q01995

22.5

8.88

329

14

+

 

Destrin alpha

   

309

17

+

209

Actin-related protein 2/3

O15511

16.2

5.47

245

14

+

 

complex subunit 5

      

243

Retinoic acid-binding protein II, cellular

P29373

15.6

5.43

208

19

+

 

14 kDa phosphohistidine phosphatase

Q9NRX4

13.8

5.65

105

3

+

303

Calgizzarin (S100A11)

P31949

11.7

6.56

164

10

+

 

Cytochrome c oxidase polypeptide Vib

   

64

4

+

324

Ubiquitin

P62988

8.56

6.56

203

17

+

  1. The identified proteins and their corresponding MASCOT scores obtained by 2D-PAGE separation combined with MALDI-TOFMS PMF and nano-HPLC-ESI-MS/MS peptide sequencing. Spot numbers corresponds to data in Fig. 3 with changes in their expression reported in Table 2. The corresponding transcripts (*) of the proteins identified by microarray in leiomyomas and myometrium at p ≤ 0.05 and 2-fold cutoff are shown as + in the right hand side column. The Accession numbers, theoretical MW and pI are according to and calculated from Swiss-Prot.