Figure 4
FPr mRNA expression in porcine corpora lutea-microvascular endothelial cells (pCL-MVECs) freshly isolated (Fresh) and cultured for different times (overnight-O/N; confluence-Conf.) and in porcine Aortic Endothelial Cells (pAECs). The confluent stage of the MVECs was reached in the presence (+) or absence (-) of hormonal treatment (H = P4 [10-6M] + E2 [10-8 M]). The results are presented as Delta Ct (HPRT Ct - FPr Ct). The data represent mean ± SD. Different letters indicate statistically significant differences (P < 0.05).