Figure 1

Representative images of mid-uteal-phase porcine corpus luteum-microvascular endothelial cell (ML-p pCL-MVEC) cultures. A) pCL-MVECs after 2 days of culture with beads still attached (phase contrast microscope ×200). B) the pCL-MVEC monolayer at confluence (day 7) (phase contrast microscope ×100). Different endothelial cell types are shown: spindle-shaped cells (black arrowhead), cobble-stone cells (white arrow), polygonal-opaque cells (black arrow), phase-dense cells (white arrowhead). C) Immunostaining for FVIII antigen (epifluorescence microscope ×100).