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Table 1 Primary antibodies used in this study.

From: Plasma membrane receptor mediated MAPK signaling pathways are activated in human uterine cervix at parturition

Antibody Specificity Dilution Sources
c-fos (Ab-2) Polyconal (PC05) Amonio acid residues 4–17 of human c-Fos 1:40 Oncogene Research Products, Cambridge, MA
c-Jun (Ab-2) Polyclonal (PC07) Amonio acid residues 91–105 of human c-Jun 1:40 Oncogene Research Products, Cambridge, MA
Phopho-specific P44/42 MAPK polyclonal Ab (9101S) Amonio acid residues 91–105 of human p44 MAP kinase, detects p42 and p44 MAPK (Erk1 and Erk2), only when activated by phosphorylation at Thr 202 and Tyr 204 1:250 New England Biolabs, Inc. Beverly, MA
P44/42 MAPK polyclonal Ab (9102) Amonio acid residues 345–358 of rat p42 MAPK, detects total MAP kinase (Erk1 and Erk2) levels 1:1000 New England Biolabs, Inc. Beverly, MA
Phospho-p38 MAPK polyclonal Ab (9211S) A synthetic doubly phosphorylated peptide (Thr 180/Tyr 182) of human p38 MAPK 1:500 New England Biolabs, Inc. Beverly, MA
p-JNK (G-7) Monoclonal AB (sc-6254) A short aa sequence containing phosphorylated on Thr-183 and Tyr-185 of JNK1 of human, reacts to phosporylated JNK 1–3 1:250 Santa Cruz Biotechnology, Inc. USA
JNK2 (D-2) Monoclonal AB (sc-7345) Full lengh (aa 1–424) human JNK2, reacts to JNK1-3 1:250 Santa Cruz Biotechnology, Inc. USA