Rxfp1 and Rxfp2 transcripts are present throughout the reproductive tract of the male rat. [A] Agarose gel electrophoresis with Rxfp1 (left panel, 452 bp product) and Rxfp2 (right panel, 775 bp product) transcripts amplified by RT-PCR from the testis (lane 1) and vas deferens (lane 2). Arrows indicate the expected product. [B] Densitometric analysis of Southern blots of Rxfp1 (left) and Rxfp2 (right) transcripts amplified by RT-PCR from various tissues of the male reproductive tract. Results are mean ± SEM of measurements with RNAs from 3 – 5 independent experiments, each with a different animal. [C] RT-PCR analysis of the presence of exon 4 in Rxfp1 transcripts (1837 bp product) in myometrium (lane M), testis (lane 1) and vas deferens (lane 2). Result is representative of 3 independent experiments, each with a different animal.