Immunofluorescence analysis of Hic-5 protein expression in the longitudinal smooth muscle layer of rat myometrium following administration of progesterone (4 mg in 0.2 ml corn oil sc) or oil (vehicle control; 0.2 ml corn oil sc). There were no consistent, marked changes in detection of Hic-5 protein in myometrial tissue sections from progesterone-treated rats compared to vehicle controls. P = progesterone. O = oil. Con = mouse IgG. Days 21-O, 22-O, d23-O (active labour), 21-P, 22-P, and 23-P represent gestational time-points. Scale bar = 50 μm.