Immunoblot analysis of Hic-5 protein expression in rat myometrium during pregnancy, labour and postpartum. (A) Representative immunoblots of Hic-5 and calponin expression. (B) Densitometric analysis illustrating no overall statistically significant increases in Hic-5 detection in the rat myometrium throughout the time course examined. Values are from 3 independent experiments (n = 3) ± SE. Days 6, 12, 15, 17, 19, 21, 22 and 23 (active labour) represent gestational timepoints. NP = non-pregnant, PP = 1 day postpartum.