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Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: Laser capture microdissection and cDNA array analysis of endometrium identify CCL16 and CCL21 as epithelial-derived inflammatory mediators associated with endometriosis

Figure 1

Laser capture microdissection. (A) Frozen section of eutopic endometrial tissue from an endometriosis patient (mid-secretory phase) stained with HistoGeneâ„¢. The epithelial glands were isolated with laser capture microdissection (LCM). (B) Tissue section following LCM demonstrating the precision in dissection and cell capture. (C) RNA yields from microdissected glands expressed as total RNA (ng) per number of gland from each tissue sample. Microdissected glandular RNA samples from control (C, n = 4) and endometriosis (E, n = 4) tissues were separately, reverse transcribed and a uterine epithelial cell-specific gene (HtrA3) was amplified using PCR to assess RNA quality (insert).

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