Table 1 Oligonucleotide primers used for quantitative real-time RT-PCR analysis
Gene | Primer | Sequence | Position |
|---|---|---|---|
ANXA1 | Forward | 5' GGCTTTGCTTTCTCTTGCTAAGG 3' | 611–633 |
(NM_175784) | Reverse | 5' TGAATCAGCCAAGTCGTCATTT 3' | 680–669 |
CTDSP2 | Forward | 5' GGCCTGGTGTCCAAGTCCT 3' | 203–221 |
(DT808814) | Reverse | 5' CAGAAAAGGGCCTTGAAGATGT 3' | 267–246 |
MSX1 | Forward | 5' TCCCTTGTTCAGCACCGC 3' | 1207–1224 |
(NM_174798) | Reverse | 5' CGGAGGACAAACCAGAGCA 3' | 1270–1252 |
HSPA1A | Forward | 5' GCAGACCCGCTATCTCCAAG 3' | 41–60 |
(NM_174550) | Reverse | 5' ACCTGAAAACGGCCCACAG 3' | 117–99 |
HSPA8 | Forward | 5' CAAGCTATGTCGCCTTTACTGA 3' | 115–136 |
(NM_174345) | Reverse | 5' GGATTCATTGCGACTTGGTTC 3' | 188–168 |
SULT1E1 | Forward | 5' CAGGATCATCTGGACAGTGTACCA 3' | 182–205 |
(NM_177488) | Reverse | 5' CCAAGTTTGCCAAAGTAATCTGAA 3' | 259–236 |
TFAP2A | Forward | 5' CCCAACGAAGTCTTCTGTTCAGT 3' | 775–797 |
(XM_875452) | Reverse | 5' ACCTTGTACTTCGAGGTGGAGC 3' | 842–821 |
TFAP2B | Forward | 5' CGAATGCCTCAATGCGTCT 3' | 1090–1108 |
(BC120374) | Reverse | 5' CCCATTTTTCGATTTGGCTC 3' | 1150–1131 |
TFAP2C | Forward | 5' GGTGTTCTCAGAAGAGCCAAGTC 3' | 1016–1038 |
(BC120401) | Reverse | 5' GACATAGGCAAAGTCCCGAGC 3' | 1186–1166 |
GAPDH | Forward | 5' AAGGCCATCACCATCTTCCA 3' | 178–197 |
(U85042) | Reverse | 5' CCACTACATACTCAGCACCAGCAT 3' | 253–230 |